Investor Information

Potential Applications

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Protection From Ionizing Radiation

NIT’s patented intellectual property in NA has been tested for its ability to reduce ionizing radiation such as X-Rays and Gamma Rays. Although ionizing radiation is helpful in curing cancer, it is also harmful to personnel and equipment adjacent to the machines creating the rays. NA has been shown to reduce the level of the ionizing radiation energy by absorbing the rays hitting the NA and reemitting it as harmless infrared photons into the atmosphere.

This provides NIT with an opportunity to use the unique ability of its product line to provide effective solutions to reduce the impact of radiation, thus creating a market for protecting government and institutional public buildings, hospitals, clinics, dentist and doctor’s offices; anywhere there is use of ionizing radiation. Additionally, one of the largest terrorism threats to the US is a dirty nuclear bomb, which serves to contaminate and kill people with radiation. US civilian agencies and the military consider radiation protections against this threat as one of their highest priorities.

The US government has billions of dollars set aside for effective protections against chemical, biological, radiological, and nuclear threats, which the government abbreviates “CBRN”. CBRN and space applications represent immediate business opportunities for NIT.

NASA Studies Cosmic Radiation to Protect High-Altitude Travelers

CBRN

An initial potential market for NIT is to immediately respond to requests for products from civilian and military agencies of the government for NA to be used in CBRN applications. Funding from these agencies is available across the board, from early stage product application development to stock-piling production. NIT has on its staff and advisory board successful high level executives with over 140 years’ experience in interfacing with and marketing to large government agencies and military programs. NIT intends to this experience as a springboard for its staff to aggressively market its products in the CBRN market.

NIT has identified high-level advisory personnel associated with civilian government emergency agencies, military, CDC, and military sponsored research with such institutions as CIMIT in Boston that have expressed interest in NIT’s technology as it relates to CBRN.

Bio-Threat Protection

Bio-threat protection is one potential application for NA. Air is a great transmitter of micro-particles, nerve agents, resistant germs, and biological threats that can cause harm to humans and animals alike. Filtering is the common method used to clean particulate matter out of the air. However, many threats are not particulate in form and filters must be augmented with capturing agents with a high affinity to the particular toxin of interest. NA is unique in having shown a high affinity to substances that are toxic to DNA. High-efficiency particulate air or HEPA is a type of air filter commonly used in areas as diverse as vacuum cleaners to operating rooms in hospitals. They are also highly recommended for relieving allergy triggers.

Industrial Paint Additives

Dr. Li accomplished impressive results in a laboratory comparison of the UV resistance of a quality automotive paint compared to the same paint with NITs NA, obtaining several times the paint life over the current UV protection technology. The work was performed by Dr. Li’s colleagues at Duke University’s Pratt School of Engineering. Potentially using NA to extend UV performance of such high performance paints extends to the marine, aircraft and industrial coatings markets.

NIT’s is actively working to incorporate its well proven UV technology into the window tinting film industry for buildings and automotive use. NIT has received interest at the CEO level of the two most technically advanced window film manufactures. This growing industry is so competitive, manufactures are desperate to get a technical leg up on their competitors. As with the ionizing radiation program, PTI has offered to develop the process to uniformly put the DNA/RNA into window film. It is anticipated the first DNA /RNA can be shipped to PTI with the vetted zinc molecules incorporated within three weeks of the completion of the Palm Beach Gardens lab, which will place this UV program in front technically, and potentially the first to present the opportunity for a licensing program with major manufacturers this year.

NIT’s DNA/RNA technology was tested on live DNA producing 99.99% survival rate which translates directly to review staging and skin cancer which are caused by DNA damage. Government and NIT scientists have proven high-energy UV rays are now penetrating our atmosphere primarily due to aerosols dumped into our atmosphere over many decades.

NIT’s is also investigating a dirty bomb protection program to provide both ionizing radiation and UV pro-tection for windows and exterior panels on buildings is. NIT is in preliminary discussions with the top manufacturer of bombproof/bulletproof windows, which is further in discussions with NEXTera ENERGY, the parent company of Florida Power and Light, about a product they have which is a bombproof window that actually generates solar energy. These windows pass visible light while reflecting some of the light energy to photovoltaic cells contained within the window frame. NIT offers the ability to filter out high UV-B, the major cause of skin cancer, as well as the potential for ionizing radiation from dirty bombs. The window company and NIT are in preliminary discussions about teaming. NEXTera ENERGY high level executive officials have offered to entertain funding the joint venture with their energy incentive funding set-asides.

Hospital and Medical Applications

Studies have exhibited that hospitals contain 3 times more background ionizing radiation than the normal work environments. Hospital paint containing nucleotides could reduce incidental radiation. This application would greatly reduce the financial and legal exposure that hospitals face.

Chemical Protection/First Respoonders

Finally, chemical and biological protection for first responders to an emergency is an important potential market for any government and civilian organization. Coatings containing NA on masks, scarves, screens, uniforms, any other clothing materials, as well as first responder equipment would protect and preserve the health of these vital personnel.

Ionizing Radiation Shielding Innovation

Breadboard Lead Equivalency Estimates for Two NITE Media in a Water-based Agarose Gel

Purpose: Based on measurements verifying the strong UV-blocking properties of small quantities of metal oxide particles bonded to patented RNA/DNA derivatives (herein called NITE Media), determine whether NITE Media exhibits similar attenuation properties for higher-energy radiation by calculating the lead thickness equivalent of NITE Media interposed between a detector and a radioactive source emitting 0.21 and 1.0 Me V gamma radiation.
Experimental: NIT Enterprises prepared an aqueous suspension composed of 5% DNA/RNA and 20% of a commonly available heavy rare earth (by weight). We then performed our proprietary process to bond all the available bonding sights to the rare earth (herein called NITE Media). The NITE media was then added to an aqueous infusion solvent. We then simulated putting NITE Media+solvent in a plastic by combining the mixture with the same quantity of an agarose gel-forming mixture. The gel was formed in a standard 100mm Petri dish and put into a breadboard radiation measurement system. Both empty Petri dishes (controls) and Petri dishes filled with the gelled mixture were exposed to a low-level natural
radiation source emitting both 210 keV and 1.0 MeV decay products. We achieved an 86.5% reduction in (both high energy X-ray and) gamma radiation based on the comparison of counts per minute recorded. Specifically, detector recorded an average reading of .208 mR/hr for controls vs only 0.028 mR/hr for the agarose gel treated with NITE Media in infusion solvent.
These results were verified by repeating the procedure, this time using a richer NITE Media (7.6% DNA/RNA with
15.38% Proprietary Rare Earth) additive. Detector NIT has developed a patented lightweight ionizing X-Ray and Gamma Ray absorbing technology utilizing nano-strands of DNA and RNA with their associated nucleotides. The ability to shield industrial material without using heavy metals allows this technology to be applied to a myriad of applications where shielding is not possible due to weight and size of currently existing methods. Application of NIT’s shielding technologies provides a unique tool for new and existing product enhancements in healthcare, defense, space and industrial environments.

Nucleotides are approved by the FDA for injection into the human body. NIT is in preliminary discussions with a Department of Defense agency regarding testing the technology for injection for military and first responder applications. Government agencies have significant budget dollars set aside for applications which include radiation shielding for structures, vehicles, electronics, and aerospace applications.

As shown in the example below, on the sides of the DNA/RNA ladder, there are unique bonding sites where NIT uses a patented process to bond heavy metal or rare earth Nano-particles, which effectively shield the ionizing radiation of X-Rays and Gamma Rays. The illustration below shows a DNA ladder with the UV absorbing nucleotides as the rungs of the ladder and the ionizing radiation absorbing Nano-particles bonded to the sides of the ladder. Millions of these Nano sized absorbing structures are then bonded into macro-structures such as a coating.

Preparation

The siloxane glass coating was catalyzed and the nucleotide Nano Structure were added to the coating with a shearing disperser, at the weight ratio of 126grams of Nano-DNA nucleotides to 500 grams of EcoSmart ECT-1600 glass coating in the liquid state with a solids content of approximately 15%. The coating was then poured into clear plastic petri dishes in two dried thickness; approximately 0.030 inches (0.8mm), and approximately 0.067 inches (1.7mm).

NIT has extensive positive data on its nucleotide micro-structures in UV-B and UV-C. Documentation of initial ionizing radiation testing is featured on the adjacent page. Zinc was used in our demonstration, however Zinc may not be the optimum absorber of choice. The Zinc Nano-particle version was readily available for initial testing as it is used in the UV Sunblock implementation of the technology.

NIT’s successful initial Gamma Ray tests illustrated on the adjacent page were conducted by Borg Performance Coatings, LLC, located in Daytona Beach, Florida. Borg’s Chief Investigator is Mr. Bo Gimvang who is a Chemist
and Chemical Engineer with worldwide experience. Mr. Gimvang prepared the coatings and samples for both the X-Ray and the Gamma Ray tests. The initial X-Ray tests also illustrated on the adjacent page were performed by Michael Latimer, an Assistant Professor at the Palm Beach State College department of Radiography.

NIT will be seeking government and industry funding for development of a continuing program to optimize and better quantify the performance of its ionizing radiation nucleotides. More definitive data with a variety of metals and rare earths of interest will significantly increase effectiveness over Zinc. Also, during the collection of quantification data, both the levels of radiation (i.e. space vs. nuclear explosion) for which the Nano-absorber is effective, and the longevity will be explored.

Testing for X-RAYS Using A Standard Diagnostic X-Ray Machine:

Two petri dishes at 0.030 inches and 0.067 inches, and a plug of dried coating at approximately 0.40 inches were placed in an array to be irradiated with a lithium glass control which completely blocks X-Rays. The X-RAY machine was calibrated to give a .005 rem dose. The glass and the 0.40 plug displayed no visible penetration of X-RAYS and the smaller thickness petri dishes both showed reasonable blockage.

Does NIT DNA/RNA Lose It’s Effectiveness Over Time?

NIT’s predecessor company which was purchased, developed and ISO level documented a DNA/RNA based Sunblock and Anti-aging cream, and then manufactured an initial run of product in December of 2008. It was from this batch that a nuclear measurement indicated an approximate 25% reduction in Gamma Rays, despite the very low concentration of 0.2% which is 0.1 gram of DNA/RNA per 50ml of cream.

This measurement indicated the nucleotide molecules in the DNA/RNA not only absorbed and transmuted UV, but also the high energy photons of X-Rays and Gamma Rays. It was from this work wherein Dr. Yin-Xiong Li filed for a patent to substitute heavy metals for the zinc Nano-particles attached to the Sunblock DNA/RNA.

Subsequent measurements taken by NIT scientists and Palm Beach State College Professor Michael Latimer M.S.R.S., R.T.(R), who is the President Elect and Board Member of the American Society of Radiologic Technologists, confirmed the actions of the DNA/RNA into high energy photons.

In order to prove the DNA/RNA energy transfer actions do not decline with age. A sample of the over 7 year old original cream was tested by Professor Latimer to show it retained significant effect despite the very low 0.2% concentration of the DNA/RNA.

The cream under test was put on a gauze pad approximately 1/16 inch thick .Using a Cobalt 60 source, the cream reduce the Gamma count from approximately 850 mR/hr to 650mR/hr. which is a 24.5% reduction; quite impressive for 0.1 gram in 50 ml and not optimized with heavy metals. The gauze was also X-Rayed and showed attenuation effect.

Absolutely you DNA/RNA sunblock manufactured 12/2008 was applied to a gauze pad

The expiration date stamped 12/10 is two years from the manufacturing date.

A digilert-200 geiger counter was used for the gamma measurements

A cobalt-60 one micro-curie source was used for for all high energy photons

The cream on the gauze clearly show up in this X-RAY showing effect for all high energy photons

Summary Conclusion

The cream tested was optimized for high UV-B from 300nm down through UV-C, which are low energy photons compared to X-Rays and Gamma Rays. The formula used zinc Nano-Particles instead of heavy metals bonded to the phosphate sites on the DNA/RNA ladder. And the concentrations for UV are at 0.2% to give 99.99% protection from UV photons.

The Gamma and X-Ray tests indicates after over 7 years, high energy photons are still being negated. NIT is entering an optimization program for the DNA/RNA with 15 heavy metals to perfect lightweight X-Ray and Gamma Ray shielding.