Investor Information

DR. Yin-Xiong Li, M.D., PHD.

is an esteemed geneticist and invented a process to harvest stem cells from human urine and re-educate the cells to be stem cells used to grow other human organs. Dr. Li has developed a technique to multiply and store the cells for future use and is the developer of the products distributed by NIT Enterprises. His technologies and ingredients are patented; he is a world-renowned physician and geneticist with a stellar background and track record in basic medical science research including the fields of cellular, molecular and developmental biology, biochemistry and genomics, as well as clinic research experience in cardiology, gastroenterology and neonatology. Recipient of the Fogarty Fellowship, Dr. Li.’s prestigious career includes Duke, Vanderbilt, as well as the NIH.

Education, Positions & Employment

EDUCATION

1979-1985 – Hunan Medical College, Medicine, MD. (Equivalent)

1987-1991 – Peking Union Medical College, Ph.D. Molecular Biology and Biochemistry

POSITIONS AND EMPLOYMENT

1992-1994 – Research Scientist, Vanderbilt University Medical Center

1993-2001 – Research Scientist, Medical College of Georgia

2001-2004 – Assistant Professor, Dept. of Pediatrics and Cell Biology, Duke University Medical Center

2004-2010 – Assistant Professor, Pediatrics and Cell Biology, Dept. of Medicine (Gastroenterology and Cardiology), Duke University Medical Center

2010-2011 – Chief Technology Officer and Vice President, SMT Inc.

2012-Present – Professor, Director, Institute of Public Health
– Chief Medical Officer, Chinese Academy of Sciences, Guangzhou Institutes of Biosciences and Heath;
– Vice President, Fuda Cancer Hospital Guangzhou, Affiliated hospital of GIBH-CAS

Professional Experience

1985 – Highest Honors, Outstanding M.D. Graduated Student Hunan Medical University

1987 – Award of Outstanding Young Scientist, Issued by Chinese Academy of Medical Sciences

1991 – International Young Scientist Travel Award in June. Issued by the United Nations of Education, Science and Culture Organization (UNESCO).

1992 – Chief of the National Molecular Biological Techniques Training Center, CAMS

1992 – Award for “Outstanding Correspondent” of the J Chinese Academy of Medical Sciences issued by the Chinese Academy of Medical Sciences

1992-1994 – NIH Fogarty international research fellowship award

1996 – National Science & Technology Progress Prize issued by: National Health Ministry, People’s Republic of China

1996 – National Excellence Educational Publishing Prize issued by: National Education Ministry, National News and Publication Agency, People’s Republic of China

1999 – Designated as an “Alien with Extraordinary Ability in Science and Education” by the US Immigration and Naturalization Service

2004 – ChunHui Program Scholar awarded by Ministry of Education People’s Republic of China

2005-2008 – American Heart Association, Study Section, Cellular and Molecular Biology

2006 – Duke Stanback Award for environmental factors, stem cell and tumorgenesis research

2007-Present – Regular scientific reviewer of the Journal of Gastroenterology; The Journal of Proteomics; Hepatology; Cell Regeneration

2008 – NIH Invited Keynote Speaker

2009 – NIH Study Section, Challenge Grant Editorial Panel 18, Center for Scientific Review Special Emphasis Panel (ZRG1 DKUS A58, Liver stem cell study)

2010 – NIH Study Section, NIAAA Special Emphasis Panel (ZAA1, DD 01, Alcohol center grant)

2011 – Honorary Chairman, Macau Charity Anti-Cancer Association

2008 – NIH Invited Keynote Speaker

2012 – National Natural Science Foundation, China, Grant application reviewer of genetics and bioinformatics, cell biology, developmental biology and reproductive biology

2012 – Evaluation Experts of the National Science and Technology Awards, China

2012 – Director, National Strategic Plan of 2020 in Biomedicine and Health, CAS

2013 – Adjunct Professor, The Chinese University of Hong Kong Honor

Books

Lu Shendong and Li Yin-Xiong. Editor, Modern Experimental Technology of Molecular Biology.(A textbook for undergraduate and graduate in China).
National College Publishing, Inc. Beijing, China (1993). Second Edition (1995). Third Edition (2000).

Original Scientific Contributions Of Major Significance In The Field

1. First person who discovered the cell death inhibitor gene DAP5. This gene is specifically expressed in premigratory cardiac neural crest stem cells which support heart and craniofacial development. NIG Gene Database accession Number: AF093110

2. Developed an induced promoter DNA construct making antisense RNA targeted to wnt-1 oncogene, which significantly block the mammary tumor cell growth in mice. This work was praised by Nature Biotechnology in a review on gene therapy for cancer. (Nature Biotechnology April 1999, Volume 17, Number 4, Page 403-404.) In this international highly prestigious journal, Dr. Aris Persidis, the vice president for business development at Argonex Inc., wrote a review on antisense therapeutics research. In this review, the history and current state of antisense therapeutics programs to treat diseases ranging from cancer to AIDS were discussed. Dr. Persidis referred to Dr. Yin-Xiong Li’s work as a significant step toward the application of antisense treatment for virally induced cancer. The work referred to was published in Virology on March 1999, Volume 255, Page 138-149.

3. USA patent: Li Yin-Xiong, Nucleic Acid Filter For Tumorigenic Factors. USA Pending Number: 08939,858 USA, 2000. This patent describes a number of materials and methods to prevent the deleterious effects of DNA damaging agents, which are potent carcinogens. In particular, it presents a revolutionary breakthrough for sunscreen protection. This new sunscreen contains a component which filters out DNA damaging UV light much more efficiently than any previously described product. It also establishes an important new concept and standard for determining UV light induced DNA damage, the GPF (Gene Protection Factor). GPF more accurately reflects the protection efficiency of the sunscreen than does the classical SPF standard (Sunlight Protection Factor), which is determined by how well the sunscreen prevents the change in skin redness induced by UV light.

4. USA patent application: Li Yin-Xiong, Farrell, Michael and Margaret L. Kirby (1999). Double stranded blocks specific gene expression in muticellular settings in vivo and in vitro. This patent describes a novel method for attenuating the expression of targeted genes in tissue explants and in muticellular vertabrate embryos using double-stranded RNA. The ability to attenuate the expression of targeted genes in muticellular vertabrate tissues has broad implications for future medical applications. These may include anti-viral agents, anti-tumor agents, and other therapeutics that are designed to block the expression of specific genes or specific gene alleles, such as the autosomal dominant allele that causes Huntington’s chorea.

5. “Modern Experimental Technology of Molecular Biology”, 1993. Second Edition (1995). Third Edition (2000). National College Publishing Inc., Beijing, China. National Publishing Book Number: ISBN 7-04-004571-0/Q.215. This is a textbook for undergraduate and graduate students in Molecular Biology. Two editions of this book have been published. This is a textbook for undergraduate and graduate students in Molecular Biology. Two editions of this book have been published. This textbook has been used at more than 200 Universities in China including medical colleges, agricultural colleges, and in biology programs at universities.

6. Proposed new standard for cancer cell classification based on molecular oncogenesis pathology. This work was published in Journal of Experimental and Clinical Immunology, 2: 46-49, 1990. Two of the most popular media outlets in China have covered this story. The China Central TV station broadcast a story about this work at 9:00PM on November 15, 1989. The Peking People’s Radio Station broadcast a story about this work at 3:00PM on November 15, 1989. News programs at both stations reported that the work of Dr. Li Yin-Xiong and Dr. Shi-Dong Liao on a new molecular pathological classification of cancer cells, which made a significant step toward improving diagnosis and treatment of various tumors.

7. Successfully developed an RNA amplification approach to amplify RNA from Laser Capture. Microdissected few targeted cells and apply to oligonucleitids mrcroarray research. This provides a great potential to establish precise gene expression profile for any cell or tissue which involved interesting biological event or disease. 2001.

8. Invented a way to isolate and purify adult liver stem cells, and ten of these cells can rescue on damaged liver regeneration, 2005.

9. The molecular signaling defects result in stem cell developmental abnormalities, 2007. Discovered that alcohol-induced birth defects are rooted in small molecular alternation of cholesterol modification of a key protein, Shh. A breakthrough of molecular mechanism of alcohol-induced birth defects was published online in January 22, 2007 with a Press Release on Nature.com. In the first week after online publish, journalists from top five scientific magazines and over 100 websites reported the finding. These journals are: Science Daily, Nature.com, New Scientist (USA), Chemistry and Industry (England), Theheart.com (England), Daily Telegraph (London), Newsweek (Russia). This discovery has a profound impact on prevention and diagnosis of alcohol-induced birth defects and adult tissue damages.

10. Discovered cell signaling molecules, 2009. Three very important natural components have been found that function as nutrients for Adult Stem Cell; five components have been identified as signaling molecular among gastroenterological track and body tissues to regulate food intake and energy homeostasis. It holds a great potential for contorting obesity, hypotension, and diabetes. Dr. Li’s new concept “Stem Cell Nutrient” and related technology is monumental for leading adult stem cell based health care and clinical practice in the coming years. This technology will have a major impact in anti-aging, organ regeneration, and prevention of alcohol related disease.

Selected Peer-Reviewed Publications

Li Y-X, Li, SO. DNA methylation and gene regulation research of oncogene c-myc and differentiation enzyme genes CPS1. Paper Collection of Young Scientist of Chinese Academy of Medical Sciences 2:211-217, 1988

Li Y-X. Cancer cells possess the potential for speciation. J Exp Clin Immunol 2:46-47, 1990

Hong ZT, Li Y-X. Effects of c-myc antisense RNA on cell growth and biological macromolecular biosynthesis of NIH/3T3 cells and gastric cancer cell lines. J China-Japan Friendship Hospital. 6:235-238, 1992

Robinson-Benion C, Li Y-X, Holt JT. Gene transplantation: combined antisense inhibition and gene replacement strategies. Leukemia. 8:152-155, 1994. PMID: 8152283

Li Y-X, Fan M, Zhang J, and Liang ZQ. Expression of antisense constructor reverses the tumorgenesis phenotype in HL60 cell line. Chinese Journal of Cancer Biotherapy. 2(1) 34-38, 1995

Li Y-X, Fan M, Zhang J, and Liang ZQ. Expression of c-myc gene and biosynthesis of biological macromolecules in antisense transfectant HLR60-9. Chinese National Journal of Cancer Research (Chung Hua Chung Liu Tsa Chih) 18:16-19, 1996

Li Y-X, Papkoff J, Sarhar NH. Antisense downregulation of a mouse mammary tumor virus activated protooncogene in mouse mammary tumor cells reverses the malignant phenotype. Virology 255:138-149, 1999. PMID: 10049829

Farrell M, Waldo K, Li X-Y, Kirby ML. A novel role for cardiac neutral crest in heart development. Trends Cardiovasc Med 9:214-20, 1999. PMID: 10881754

Li Y-X, Farrell MJ, Liu RP, Mohanty N, Kirby ML. Double-stranded RNA injection produces null phenotypes in zebrafish. Development Biology. 217:394-405, 2000. PMID: 10625563

Chatterjee B, Li Y-X, Zdanowicz M, Sontag JM, Chin AJ, Kozlowski DJ, Valdimarsson G, Kirby ML, and Lo CW. Analysis of Cx43x1 promoter function in the developing zebrafish embryo. Cell Communication and Adhesion 8:289-92, 2001. PMID: 12064604

Kirby ML, Lawson A, Stadt HA, Kumiski DH, Wallis KT, McCraney E, Waldo KL, Li Y-X, and Gary C. Schoenwolf. Hensen’s node gives rise to the ventral midline of the foregut: implications for organizing head and heart development. Developmental Biology 253:175-188, 2003. PMID: 12645923

Li Y-X, Zdanowicz M, Young L, Kumiski D, Leatherbury L, and Kirby ML. Cardiac neural crest in zebrafish embryos contributes to myocardial cell lineage and early heart function. Development Dynamics 226:540-550, 2003. PMID: 12619138

Li Y-X, Kirby ML. Coordinated and conserved expression of alphoid repeat and alphoid repeat-tagged coding sequences. Developmental Dynamics 228(1):72-81, 2003. PMID: 12619138

Wilkbanks AM, Fralish GB, Kirby ML, Barak LS, Li Y-X* and Caron MG*. Arrestin 2 regulates zebrafish development through the sonic hedgehog pathway. Science. 306:2264-2267, 2004. (*co-correspondence authors). PMID: 15618520

Sicklick JK, Li Y-X, Chio S, Qi Y, Chen W, Bustamante M, Huang J, Zdanowicz M, Camp T, Torbenson MS, Rojkind M, and Diehl AM. Role for Hedgehog signaling in hepatic stellate cell activation and viability. Lab Investigation. 85:1368-80, 2005. PMID: 16170335

Sicklick JK, Li Y-X, Jayaraman A, Kannangai R, Qi Y, Vivekanandan P, Ludlow JW, Owzar K, Chen W, Torbenson MS, Diehl AM. Dysregulation of the Hedgehog pathway in human hepatocarcinogenesis. Carcinogenesis. 2005 Dec 8; [Epub ahead of print], 27:748-57, 2006. PMID: 1633918

Sicklick JK, Li Y-X, Melhem A, Schmelzer E, Zdanowicz M, Huang J, Caballero M, Fair JH, Ludlow JW, McClelland Re, Reid LM, Diehl AM. Hedgehog signaling maintains resident hepatic progenitors throughout life. Am J Physiol Gastrointest Liver Physiol. 27:748-57, 2006. PMID: 16322088

Hutson ML, Zhang P, Stadt HA, Sato A, Li Y-X, Burch J, Creazzo TL, Kirby ML. Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx. Developmental Biology, 295(2):486-97, 2006. PMID: 16765936

Sicklick JK, Choi SS, Bustamante M McCall SJ, Hernandez-Paz S, Huang J, Li Y-X, Rojkind M, and Diehl AM. Evidence for Epithelialmesenchymal transitions in adult liver cells. AM J Physiol Gastrointest Liver Physiol. 291(4):G575-83, 2006. PMID: 16710052

Suzuki A, McCall S, Choi SS, Sicklick JK, Huang J, Qi Y, Zdanowicz M, Camp T, Li Y-X and Diehl AM. Interleukin-15 increases hepatic regenerative activity. J Hepatol. 45(3):410-8, 2006. PMID: 16781000

Choi SS, Sicklick JK, Ma Q, Yang L, Huang J, Qi Y, Chen W, Li Y-X, Goldschmidt-Clermont PJ, and Diehl AM. Sustained activation of Rac1 in hepatic stellate cells promotes liver injury and fibrosis in mice/ Hepatology. 44(5):1267-77, 2006. PMID: 1705826

Li Y-X*, Yang HT, Danowicz M, Sicklick JK, Qi Y, Camp T, and Diehl AM. Fetal Alcohol Exposure Impairs Hedgehog Cholesterol Modification and Signaling. Lab Invest. 87(3):231-40, 2007. (Presented as the Journal cover picture and with an Editorial Press Release) (*correspondence authors). PMID: 17237799

Omenetti A*, Yang L*, Li Y-X, McCall SJ, Sicklick JK, Huang J, Choi S, Suzuki A, and Diehl AM. Hedgehog mediated mesenchymal-epithelial interactions modulate hepatic response to bile duct ligation. Lab Invest. 87(5):499-514, 2007. (*Equally contribution). PMID: 17334411

Jung Y, McCall SJ, Li Y-X, and Diehl AM. Bile Ductules and Stromal Cells Express Hedgehog Ligands and/or Hedgehog Target Genes in Primary Biliary Cirrhosis. Hepatology. 45(5):1091-6, 2007. PMID: 17464985

Yamaguchi K, Yang L, McCall S, Huang J, Yu XX, Pandey SK, Bhanot S, Monia BP, Li Y-X, Diehl AM. Inhibiting triglyceride synthesis improves hepatic steatosis but exacerbates liver damage and fibrosis in obese mice with nonalcoholic steatohepatitis. Hepatology. 45(6):1343-6, 2007. PMID: 17476695

Yamaguchi K, Yang L, McCall S, Huang J, Yu XX, Pandey SK, Bhanot S, Monia BP, Li Y-X, Diehl AM. Diacylglycerol acyltranferase anti-sense oligonucleotides reduce hepatic fibrosis in mice with nonalcoholic steatohepatitis. Hepatology 47:625-35, 2008. PMID: 1802272. PMC2196213

Hua Mao H, Diehl AM, Li Y-X. Sonic Hedgehog Ligand Partners with Caveolin-1 for Intracellular Transportation. Lab Invest. 89:290-300, 2009. PMID 19139721. PMC2647995

PatentsSelected peer-reviewed publications (in chronological order)

What is UV?

As wavelengths grow shorter, their frequency increases in proportion, and rays and photons become more harmful to DNA, cell structure, and outdoor exposed surfaces. Although not having enough energy to ionize atoms,
UV (Ultraviolet Radiation) does have the energy to alter chemical bonds,
which is very damaging to DNA, paints, fabric, and plastics.

What Is Harmful Radiation?

UV-A Starts above visible lights and spans to UV-B region. UV-A is not filtered by the atmosphere and is less energetic than the rest of the UV spectrum and thus less damaging to DNA and outdoor surfaces. However, the FDA requires sunblocks to protect against UV-A and the part of UV-B that passes through the atmosphere.

UV-B spans the part of the spectrum where the earth’s atmosphere absorbs only about half of the UV-B wavelengths. The part of the UV-B that is not absorbed by the atmosphere is primarily the part attributed to causing sunburn. The point where absorption stops is critical as the susceptibility to DNA damage and skin cancer skyrockets when damaged to the ozone layer allows the short wavelengths (high reguency) part of UV-B to leak through to the earths surface. Current damage done to the ozone layer is allowing these damaging frequencies to leak through the atmosphere with large weather fronts even at mid-altitudes. UV-B, being the highest energy UV which penetrates the atmosphere, does the most damage in oxidizing and fading outdoor surfaces.

UV-C generated by the sun is completely absorbed by the earths atmosphere. With the exception of lightning strikes of short duration the only prolonged sources are man-made in special bulbs or electric arcs. UV-C is deadly to cells as evidenced in the use of UV-C for sterilization lights and the fact that welders experience the highest rate of skin cancer.

X-Rays radiation generally occurs from electrons surrounding the atom and they are second only to Gamma Rays in photon energy. X-Rays are so commonly used in medical and industial imaging, they are well known to the general public. However, prolonged or high dosage exposure to X-Rays can cause carcinogenesis, mutations and death to the DNA in cells.

Gamma Rays are the highest photon energy in the electromagnetic spectrum having the shortest wavelengths. Gamma Rays are generally emitted from the nucleus of the atoms. Thus, they are generated by nuclear reactions, such as in the sun or other nuclear reactions in the cosmos, or by radioactive decay as used in radiation sources reactors or in man-made medical radiation and industrial equipment. Their effectiveness in killing cells is evidenced by their common use in killing cells in cancer tumors. Most gamma radiation from space is filtered out by the atmosphere. The amount of gamma radiation exposure on a transcontinental flight at high altitude is comparable to having a medical X-Ray. Gamma radiation in space is significant and a hazard to astronauts and electronics.